Importance of the evaluation of lactobacillus plantarum atcc 8041® microencapsulate for the agroindustrial sector
Keywords:
ETA, LAB, Lactobacillus plantarum, Prebiotics, Probiotics, Campylobacter jejuni.Abstract
The inhibitory effect of microencapsulated Lactobacillus plantarum and its viability under simulated gastrointestinal conditions were evaluated. Reconstitution, sowing and adjustment of the inoculum were carried out; antibiogram; fermentation kinetics; determination of peptides, amino acids and lactic acid in the supernatant; resistance to temperatures of 37 and 45°C; micro-encapsulation; study at simulated gastrointestinal conditions of the microencapsulate after 90 days of storage; and production of exopolysaccharides (EPS). The results indicate inhibitory action of the L. plantarum strain against the pathogenic bacteria; exponential phase at 15 hours (MRS culture medium) and at 18 hours (PRO culture medium), with the kinetic parameters in the exponential phase as follows: protein production 2.43mg/L (MRS) and 4, 86mg/L (PRO); sugar consumption 4.96 mg/L (MRS) and 7.68 mg/L (PRO); acidity percentage 1.32% (MRS) and 1.75% (PRO); pH 4.07 (MRS) and 3.86 (PRO); results of the study and analysis of the microencapsulation: 100% viability; efficiency 84.64%; humidity 4.0%; solubility 99.8%; wettability 2 min 22 seconds; water activity 0.617 and particle size between 2.10µm and 5.28µm. It was concluded that microencapsulated L. plantarum presented inhibitory properties against the pathogenic strain and has in vitro adherence.
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