Importance of the evaluation of lactobacillus plantarum atcc 8041® microencapsulate for the agroindustrial sector
Published 2023-12-15
Keywords
- ETA, LAB, Lactobacillus plantarum, Prebiotics, Probiotics, Campylobacter jejuni.
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Abstract
The inhibitory effect of microencapsulated Lactobacillus plantarum and its viability under simulated gastrointestinal conditions were evaluated. Reconstitution, sowing and adjustment of the inoculum were carried out; antibiogram; fermentation kinetics; determination of peptides, amino acids and lactic acid in the supernatant; resistance to temperatures of 37 and 45°C; micro-encapsulation; study at simulated gastrointestinal conditions of the microencapsulate after 90 days of storage; and production of exopolysaccharides (EPS). The results indicate inhibitory action of the L. plantarum strain against the pathogenic bacteria; exponential phase at 15 hours (MRS culture medium) and at 18 hours (PRO culture medium), with the kinetic parameters in the exponential phase as follows: protein production 2.43mg/L (MRS) and 4, 86mg/L (PRO); sugar consumption 4.96 mg/L (MRS) and 7.68 mg/L (PRO); acidity percentage 1.32% (MRS) and 1.75% (PRO); pH 4.07 (MRS) and 3.86 (PRO); results of the study and analysis of the microencapsulation: 100% viability; efficiency 84.64%; humidity 4.0%; solubility 99.8%; wettability 2 min 22 seconds; water activity 0.617 and particle size between 2.10µm and 5.28µm. It was concluded that microencapsulated L. plantarum presented inhibitory properties against the pathogenic strain and has in vitro adherence.
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References
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